RET-KIF5B (Kex15Rex14) Kinase Assay Service

Activity-based RET-KIF5B (Kex15Rex14) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.

Target Overview
CAT#

RET, KIF5B (Kex15Rex14)-

Enzyme

Human KIF5B-RET (Kex15Rex14) Fusion protein

HGNC

RET-KIF5B

Synonym

CDHF12, CDHR16, HSCR1, MEN2A, MEN2B, MTC1, PTC, RET51

Substrates

Peptide substrate, [RRRRRRRRRRRVYSTDYYRLFNPS], 20 µM
ATP 10 µM

Assay Properties
Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 4.3
PP2 23
AG1478 290
EGFR/Erb Inhibitor >100,000
D-64406 115
Scientific Information

KIF5B: P33176 (http://www.uniprot.org/uniprot/P33176)
RET; P07949 (http://www.uniprot.org/uniprot/P07949)

Screening Location

Malvern, USA

Reference compound IC50 for RET-KIF5B (Kex15Rex14)