RET-NCOA4 (PTC3) Kinase Assay Service

Activity-based RET-NCOA4 (PTC3) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.

Target Overview
CAT#

-RET, NCOA4 (PTC3)

Enzyme

Human NCOA4-RET (PTC3) fusion

HGNC

RET-NCOA4

Synonym

CDHF12, CDHR16, HSCR1, MEN2A, MEN2B, MTC1, PTC, RET51

Substrates

Peptide substrate, [RRRRRRRRRRRVYSTDYYRLFNPS], 20 µM
ATP 10 µM

Assay Properties
Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 5.0
PP2 27
AG1478 330
EGFR/ErbB Inhibitor >100,000
D-64406 160
Scientific Information

NCOA4; Q13772 (http://www.uniprot.org/uniprot/Q13772)
RET; P07949 (http://www.uniprot.org/uniprot/P07949)

Screening Location

Malvern, USA

Reference compound IC50 for RET-NCOA4