RET (G810C) Kinase Assay Service

Activity-based RET (G810C) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#

RET (G810C)

Enzyme

Human RET (G810C)

HGNC

RET (G810C)

Synonym

Proto-oncogene tyrosine-protein kinase receptor ret, C-ret, CDHF12, CDHR16, HSCR1, MEN2A, MEN2B, MTC1, PTC, RET51

Substrates

Peptide substrate, RRRRRRRRRRRVYSTDYYRLFNPS], 20 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 260
PP2 50
AG1478 15,300
EGFR/ErbB Inhibitor >100,000
D-64406 3,600

Scientific Information

P07949 (http://www.uniprot.org/uniprot/P07949)

Screening Location

Malvern, USA

Reference compound IC50 for RET (G810C)

Reference compound IC50 for RET (G810C)