RET (V804M)-KIF5B Kinase Assay Service

Activity-based RET (V804M)-KIF5B kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.

Target Overview

CAT#

RET (V804M), KIF5B-

Enzyme

Human KIF5B-RET (V804M) Fusion protein

HGNC

RET (V804M)-KIF5B

Synonym

CDHF12, CDHR16, HSCR1, MEN2A, MEN2B, MTC1, PTC, RET51

Scientific Information

KIF5B: P33176 (http://www.uniprot.org/uniprot/P33176)
RET: P07949 (http://www.uniprot.org/uniprot/P07949)

Assay Properties

Substrates

Peptide substrate, [RRRRRRRRRRRVYSTDYYRLFNPS], 20 µM
ATP, 10 µM

Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 1.4
PP2 >100,000
AG1478 >100,000
EGFR/Erb Inhibitor >100,000
D-64406 320

Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Kinase Assays

Reference compound IC50 RET (V804M) KIF5B

Reference compound IC50 RET (V804M) KIF5B

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