RET (Y806N) Kinase Assay Service

Activity-based RET (Y806N) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.

Target Overview
CAT#

RET (Y806N)

Enzyme

Human RET (Y806N)

HGNC

RET (Y806N)

Synonym

CDHF12, CDHR16, HSCR1, MEN2A, MEN2B, MTC1, PTC, RET51

Substrates

Peptide substrate, [RRRRRRRRRRRVYSTDYYRLFNPS], 20 µM
ATP 10 µM

Assay Properties
Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 80
PP2 130
AG1478 6,900
EGFR/ErbB Inhibitor >100,000
D-64406 2,200
Scientific Information

P07949 (http://www.uniprot.org/uniprot/P07949)

Screening Location

Malvern, USA

Reference compound IC50 for RET (Y806N)