Activity-based RIPK1 kinase assay for compound screening and profiling via ADP-Glo kinase assay.
Target Overview
CAT#
RIPK1 (ADP-Glo)
Enzyme
Human RIPK1
HGNC
RIPK1
Synonym
RIP1, RIP
Scientific Information
Q13546 (http://www.uniprot.org/uniprot/Q13546)
Assay Properties
Substrates
ATP, 10 µM, Km (100 µM), 1 mM, Cofactor MnCl2
Reaction
ATP = ADP + Pi
Readout
ADP-Glo Detection
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| GSK2982772 (10 µM ATP) | 10.2 |
| Staurosporine (10 µM ATP) | 47.7 |
| Staurosporine (100 µM ATP) | 81.9 |
| Staurosporine (1 mM ATP) | 592.4 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound inhibition for RIPK1
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