Activity-based RIPK2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
RIPK2
Enzyme
Human RIPK2
HGNC
RIPK2
Synonym
CARD3, CARDIAK, CCK, GIG30, RICK, RIP-2, RIP2
Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 240 |
| Ro 31-8220 | >20,000 |
| GW 5074 | 290 |
Scientific Information
O43353 (http://www.uniprot.org/uniprot/O43353)
Screening Location
Malvern, USA
Reference compound IC50 for RIPK2
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