Activity-based RIPK4 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
RIPK4
Enzyme
Human RIPK4
HGNC
RIPK4
Synonym
Receptor interacting protein kinase 4, ANKK2, ANKRD3, DIK, MGC129992, MGC129993, PKK
Substrates
Histone H2A, 10 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 380 |
| Ro 31-8220 | >100,000 |
| GW5074 | 12,000 |
| H-89 | >100,000 |
| Rottlerin | >100,000 |
Scientific Information
P57078 (http://www.uniprot.org/uniprot/P57078)
Screening Location
Malvern, USA
Reference compound IC50 for RIPK4
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