Activity-based RIPK5 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
RIPK5
Enzyme
Human RIPK5
HGNC
DSTYK
Synonym
SgK496, SHIK
Substrates
MBP (Myelin Basic Protein), 10 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 120 |
| Ro 31-8220 | 8,000 |
| GW 5074 | 19,000 |
| Rottlerin | 20,000 |
Scientific Information
Q6XUX3 (http://www.uniprot.org/uniprot/Q6XUX3)
Screening Location
Malvern, USA
Reference compound IC50 for RIPK5
PDF