ROCK1 Kinase Assay Service

Activity-based ROCK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
ROCK1
Enzyme
Human ROCK1
HGNC
ROCK1
Synonym
P160ROCK
Substrates
Peptide substrate, [KEAKEKRQEQIAKRRRLSSLRASTSKSGGSQK], 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine0.93
Ro 31-82203,700
GW 507411,500
H-89230
Scientific Information
Q13464 (http://www.uniprot.org/uniprot/Q13464)
Screening Location
Malvern, USA
Reference compound IC50 for ROCK1
Reference compound IC50 for ROCK1