Activity-based ROCK2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
ROCK2
Enzyme
Human ROCK2
HGNC
ROCK2
Synonym
KIAA0619, ROC2
Substrates
Peptide substrate, [KEAKEKRQEQIAKRRRLSSLRASTSKSGGSQK], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 0.2 |
| Ro 31-8220 | 1,600 |
| GW5074 | 1,800 |
| H-89 | 16 |
| Rottlerin | >100,000 |
Scientific Information
O75116 (http://www.uniprot.org/uniprot/O75116)
Screening Location
Malvern, USA
Reference compound IC50 for ROCK2
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