Activity-based RON/MST1R kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
RON/MST1R
Enzyme
Human RON/MST1R
HGNC
MST1R
Synonym
CD136, CDw136, PTK8
Substrates
Peptide substrate, [KKSRGDYMTMQIG], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 220 |
| PDGFR I-III | 25,000 |
| D-64406 | >25,000 |
Scientific Information
Q04912 (http://www.uniprot.org/uniprot/Q04912)
Screening Location
Malvern, USA
Reference compound IC50 for RON/MST1R
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