ROS1-TPM3 Kinase Assay Service

Activity-based ROS1-TPM3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
-ROS1, TPM3

Enzyme
Human TPM3-ROS1 fusion

HGNC
ROS1-TPM3

Synonym
ROS, MCF3

Substrates
Peptide substrate, [KKKSPGEYVNIEFG], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 0.50
PP2 4,400
AG1478 2,100
PDGFR I-III 44,000
D-64406 35,000
Scientific Information
TPM3: P06753 (http://www.uniprot.org/uniprot/P06753)ROS1: P08922 (http://www.uniprot.org/uniprot/P08922)

Screening Location
Malvern, USA

Reference compound IC50 for ROS-TPM3
Reference compound IC50 for ROS-TPM3

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