ROS1 (G2101C) Kinase Assay Service

Activity-based ROS1 (G2101C) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#

ROS1 (G2101C)

Enzyme

Human ROS1 (G2101C)

HGNC

ROS1 (G2101C)

Synonym

Proto-oncogene tyrosine-protein kinase ROS, Receptor tyrosine kinase c-ros oncogene 1, MCF3

Substrates

Peptide substrate, [KKKSPGEYVNIEFG], 20 µM
ATP 1 µM

Assay Properties
Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 0.1
PP2 4,400
AG1478 1,500
EGFR/ErbB Inhibitor >100,000
D-64406 9,900
Scientific Information

P08922 (http://www.uniprot.org/uniprot/P08922)

Screening Location

Malvern, USA

Reference compound IC50 for ROS1 (G2101C)

Reference compound IC50 for ROS1 (G2101C)