ROS/ROS1 Kinase Assay Service

Activity-based ROS/ROS1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
ROS/ROS1

Enzyme
Human ROS/ROS1

HGNC
ROS1

Synonym
ROS, MCF3

Substrates
Peptide substrate, [KKKSPGEYVNIEFG], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine <0.1
PP2 3,100
AG1478 2,600
PDGFR I-III >20,000
D-64406 >20,000
Scientific Information
P08922 (http://www.uniprot.org/uniprot/P08922)

Screening Location
Malvern, USA

Reference compound IC50 for ROS/ROS1
Reference compound IC50 for ROS/ROS1