RSK2 (I416V) Kinase Assay Service

Activity-based RSK2 (I416V) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
RSK2 (I416V)

Enzyme
Human RSK2 (I416V)

HGNC
RPS6KA3 (I416V)

Synonym
RSK2, HU-3, MRX19, CLS

Substrates
Peptide substrate, [KKLNRTLSVA], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine <0.1
Ro 31-8220 <0.2
GW5074 7,400
H-89 310
Rottlerin 18,000
Scientific Information
P51812 (http://www.uniprot.org/uniprot/P51812)

Screening Location
Malvern, USA

Reference compound IC50 for RSK2 (I416V)
Reference compound IC50 for RSK2 (I416V)