SARS-CoV-2 Mpro Protease Assay Service

SARS-CoV-2 Mpro Protease Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#

Mpro

Enzyme

SARS-CoV-2 Mpro

Synonym

SARS-CoV-2 Main Protease, SARS-CoV-2 3C-like Proteinase, 3CLpro

Substrates

Fluorogenic substrate [NH2-C(EDANS)VNSTQSGLRK(DABCYL)M-COOH], 5 μM

Assay Properties
Readout

Enzymatic activity

Reference Compound IC50s
Presence of 1 mM DTT Compounds IC50 (nM)
GC376 6.5
Carmofur 36,000
Ebselen >100,000
Absence of DTT Compounds IC50 (nM)
GC376 5.5
Carmofur 35
Ebselen 25
Scientific Information

Accession #QHD43415.1

Screening Location

Malvern, USA

Further information

More information can be found on our website Protease Assay Services.

Reference compound IC50 for SARS-CoV-2 Mpro

Reference compound IC50 for Sars-cov-2 mpro