SBK1 Kinase Assay Service

Activity-based SBK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
SBK1
Enzyme
Human SBK1
HGNC
SBK1
Synonym
SH3-binding kinase 1
Substrates
Peptide substrate, [LCGRTGRRNSI], 100 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine62
Ro 31-822021,000
GW 507430,000
H-89>100,000
Rottlerin>100,000
Scientific Information
Q52WX2 (http://www.uniprot.org/uniprot/Q52WX2)
Screening Location
Malvern, USA
Reference compound IC50 for SBK1
Reference compound IC50 for SBK1