SET7/9 Methyltransferase Assay Service

Activity-based SET7/9 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
SET7/9
Enzyme
Human SET7/9
Synonym
Histone-lysine N-methyltransferase SETD7, Histone H3-K4 methyltransferase SETD7, H3-K4-HMTase SETD7, SET domain-containing protein 7, SET7/9, Lysine N-methyltransferase 7, KIAA1717, KMT7, SET7, SET9
Substrates
Core Histone, 0.05 mg/mlSAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + histone L- lysine = S-adenosyl-L-homocysteine + histone [methyl-³H]-L- lysine
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
SAH84,400
Sinefugin5,200
Scientific Information
Q8WTS6 (http://www.uniprot.org/uniprot/Q8WTS6)
Screening Location
Malvern, USA
Reference compound IC50 for SET7/9
Reference compound IC50 for SET7/9