SET8 Methyltransferase Assay Service

Activity-based SET8 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
SET8
Enzyme
Human SET8
Synonym
Histone-lysine N-methyltransferase SETD8, H4-K20-HMTase SETD8, SET domain-containing protein 8, PR/SET domain-containing protein 07, Lysine N-methyltransferase 5A, KMT5A, PRSET7, SET07
Substrates
Nucleosomes, 0.05 mg/mlSAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N⁶-[methyl-³H]-L-lysine
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
SAH110,000
Sinefugin220,000
Scientific Information
Q9NQR1 (http://www.uniprot.org/uniprot/Q9NQR1)
Screening Location
Malvern, USA
Reference compound IC50 for SET8
Reference compound IC50 for SET8