SETDB1 Methyltransferase Assay Service

Activity-based SETDB1 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#

SETDB1

Enzyme

Human SETDB1

Synonym

Histone-lysine N-methyltransferase SETDB1, ERG-associated protein with SET domain, 
Histone H3-K9 methyltransferase 4, SET domain bifurcated 1, KG1T, KIAA0067, ESET, KMT1E, TDRD21

Substrates

Histone H3, 5 μM
SAM, 1 μM

Assay Properties
Reaction

S-adenosyl-L-[methyl-³H]methionine + histone L- lysine = S-adenosyl-L-homocysteine + histone [methyl-³H]-L- lysine

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
SAH 3,400
Chaetocin 9,700
Sinefungin 120,000
Scientific Information

Q15047 (http://www.uniprot.org/uniprot/Q15047)

Screening Location

Malvern, USA

Further information

More information can be found on our website Methyltransferase Assay Services.

Reference compound IC50 for SETDB1

Reference compound IC50 for SETDB1