SGK1 Kinase Assay Service

Activity-based SGK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
SGK1
Enzyme
Human SGK1
HGNC
SGK1
Synonym
SGK
Substrates
Peptide substrate, [KGSGSGRPRTSSFAEG], 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine16
Ro 31-822096
GW 50743,200
H-891,500
Rottlerin15,000
Scientific Information
O00141 (http://www.uniprot.org/uniprot/O00141)
Screening Location
Malvern, USA
Reference compound IC50 for SGK1
Reference compound IC50 for SGK1