Activity-based SGK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
SGK1
Enzyme
Human SGK1
HGNC
SGK1
Synonym
SGK
Substrates
Peptide substrate, [KGSGSGRPRTSSFAEG], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 16 |
| Ro 31-8220 | 96 |
| GW 5074 | 3,200 |
| H-89 | 1,500 |
| Rottlerin | 15,000 |
Scientific Information
O00141 (http://www.uniprot.org/uniprot/O00141)
Screening Location
Malvern, USA
Reference compound IC50 for SGK1
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