SGK3/SGKL Kinase Assay Service

Activity-based SGK3/SGKL kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
SGK3

Enzyme
Human SGK3

HGNC
SGK3

Synonym
CISK, SGK2, SGKL

Substrates
Peptide substrate, [GRPRTSSFAEG], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 236
Ro 31-8220 745
Rottlerin 2,300
Scientific Information
Q96BR1 (http://www.uniprot.org/uniprot/Q96BR1)

Screening Location
Malvern, USA

Reference compound IC50 for SGK3/SGKL
Reference compound IC50 for SGK3/SGKL