Activity-based SIK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
SIK1
Enzyme
Human SIK1
HGNC
SIK1
Synonym
SIK, MSK, SNF1LK
Substrates
Peptide substrate, [AMARAASAAALARRR], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 1.2 |
| Ro 31-8220 | 3,000 |
| GW5074 | 3,600 |
| H-89 | 29,000 |
| Rottlerin | >30,000 |
Scientific Information
P57059 (http://www.uniprot.org/uniprot/P57059)
Screening Location
Malvern, USA
Reference compound IC50 for SIK1
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