SIK1 Kinase Assay Service

Activity-based SIK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
SIK1
Enzyme
Human SIK1
HGNC
SIK1
Synonym
SIK, MSK, SNF1LK
Substrates
Peptide substrate, [AMARAASAAALARRR], 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine1.2
Ro 31-82203,000
GW50743,600
H-8929,000
Rottlerin>30,000
Scientific Information
P57059 (http://www.uniprot.org/uniprot/P57059)
Screening Location
Malvern, USA
Reference compound IC50 for SIK1
Reference compound IC50 for SIK1