Activity-based SIK3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
SIK3
Enzyme
Human SIK3
HGNC
SIK3
Synonym
L19, QSK
Substrates
Peptide substrate, [AMARAASAAALARRR], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 1.1 |
| Ro 31-8220 | 3,900 |
| GW5074 | 6,800 |
| H-89 | 16,400 |
| Rottlerin | >20,000 |
Scientific Information
Q9Y2K2 (http://www.uniprot.org/uniprot/Q9Y2K2)
Screening Location
Malvern, USA
Reference compound IC50 for SIK3
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