SIK3 Kinase Assay Service

Activity-based SIK3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
SIK3
Enzyme
Human SIK3
HGNC
SIK3
Synonym
L19, QSK
Substrates
Peptide substrate, [AMARAASAAALARRR], 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine1.1
Ro 31-82203,900
GW50746,800
H-8916,400
Rottlerin>20,000
Scientific Information
Q9Y2K2 (http://www.uniprot.org/uniprot/Q9Y2K2)
Screening Location
Malvern, USA
Reference compound IC50 for SIK3
Reference compound IC50 for SIK3