SIRT5 Sirtuin Assay Services

SIRT5 Sirtuin Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.

SIRT5

Target Overview

Enzyme

Human SIRT5 (NAD+-dependent enzyme)

Synonyms

NAD-dependent protein deacylase sirtuin-5, Regulatory protein SIR2 homolog 5, SIR2-like protein 5, SIR2L5, Class III HDAC

Assay Properties

Readout

Enzymatic activity

Substrates

Fluorogenic peptide (Ac-Lys-succ-AMC), 50 μM
NAD+, 500 μM

Reference Compound IC50s
Compounds IC50 (μM)
Nicotinamide 24.2

Scientific Information

Q9NXA8 (http://www.uniprot.org/uniprot/Q9NXA8)

Screening Location

Malvern, USA

Reference Compound IC50 for SIRT5

Reference Compound IC50 for SIRT5