SMARCA2a-His Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.
Target Overview
CAT#
Tm-SMARCA2a-H
Protein
Human SMARCA2a bromodomain, His-tagged
Synonyms
BAF190B, BRM, SNF2L2
Assay Properties
Readout
Thermal stability change after ligand binding
Concentration of protein in assay
0.2 mg/ml (10.2 µM)
Concentration of compound in assay
25 µM
Apo form Tm (°C)
41,5
Reference Compound ΔTms:
PFI3 – 6.5 (Purple) No stabilization was observed in the presence of control compounds (JQ1, PFI1, CBP112, Bromosporine, SGC-CBP30, BET151, RVX-208 and GSK2801)
Scientific Information
P51531 (http://www.uniprot.org/uniprot/P51531)
Screening Location
Malvern, USA
Further information
More information can be found on our website Reader Domain Assay Services.
Melt Curve
Melt Peak
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