SMARCA2a Bromodomain Assay Service (ThermoShift)

SMARCA2a-His Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.

Target Overview
CAT#

Tm-SMARCA2a-H

Protein

Human SMARCA2a bromodomain, His-tagged

Synonyms

BAF190B, BRM, SNF2L2

Assay Properties
Readout

Thermal stability change after ligand binding

Concentration of protein in assay

0.2 mg/ml (10.2 µM)

Concentration of compound in assay

25 µM

Apo form Tm (°C)

41,5

Reference Compound ΔTms:

PFI3 - 6.5 (Purple) No stabilization was observed in the presence of control compounds (JQ1, PFI1, CBP112, Bromosporine, SGC-CBP30, BET151, RVX-208 and GSK2801)

Scientific Information

P51531 (http://www.uniprot.org/uniprot/P51531)

Screening Location

Malvern, USA

Further information

More information can be found on our website Reader Domain Assay Services

Melt Curve

Melt Curve

Melt Peak

Melt Peak