SMARCA4 Bromodomain Assay Service (AlphaScreen)

SMARCA4-His Reader Domain Assay for compound screening and profiling using the AlphaScreen assay format to measure compound binding to the target.

Target Overview

CAT#

SMARCA4-His

Enzyme

Human SMARCA4-His

Synonyms

Transcription activator BRG1, ATP-dependent helicase SMARCA4, BAF190A, BRG1, SNF2B, SNF2L4

Substrates

Histone H3 peptide (1-30) K4/18/23/27Ac

Assay Properties

Reaction

AlphaScreen Binding assay

Readout

Fluorescence

Reference Compound IC50s
Compounds IC50 (nM)
PFI-3 420
Bromosporine 45,000

Scientific Information

P51532 (http://www.uniprot.org/uniprot/P51532)

Screening Location

Malvern, USA

Further information

More information can be found on our website Reader Domain Assay Services.

Reference Compound IC50 for SMARCA4

Reference Compound IC50 for SMARCA4