SMARCA4-His Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.
Target Overview
CAT#
Tm-SMARCA4-H
Protein
Human SMARCA4 bromodomain, His-tagged
Synonyms
BRG1, BAF190A, Protein BRG-1, SNF2-beta
Assay Properties
Readout
Thermal stability change after ligand binding
Concentration of protein in assay
0.15 mg/ml (8.8 µM)
Concentration of compound in assay
25 µM
Apo form Tm (°C)
51,5
Reference Compound ΔTms:
PFI3 – 6 (Purple) No stabilization was observed in the presence of other control compounds (JQ1, PFI1, CBP112, Bromosporine, SGC-CBP30, BET151, RVX-208, and GSK2801)
Scientific Information
P51532 (http://www.uniprot.org/uniprot/P51532)
Screening Location
Malvern, USA
Further information
More information can be found on our website Reader Domain Assay Services.
Melt Curve
Melt Peak
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