SMARCA4 Bromodomain Assay Service (ThermoShift)

SMARCA4-His Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.

Target Overview




Human SMARCA4 bromodomain, His-tagged


BRG1, BAF190A, Protein BRG-1, SNF2-beta

Assay Properties


Thermal stability change after ligand binding

Concentration of protein in assay

0.15 mg/ml (8.8 µM)

Concentration of compound in assay

25 µM

Apo form Tm (°C)


Reference Compound ΔTms:

PFI3 – 6 (Purple) No stabilization was observed in the presence of other control compounds (JQ1, PFI1, CBP112, Bromosporine, SGC-CBP30, BET151, RVX-208, and GSK2801)

Scientific Information

P51532 (

Screening Location

Malvern, USA

Further information

More information can be found on our website Reader Domain Assay Services.

Melt Curve

Melt Curve

Melt Peak

Melt Peak