SMYD2 Methyltransferase Assay Service

Activity-based SMYD2 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
SMYD2
Enzyme
Human SMYD2
Synonym
SET and MYND domain-containing protein 2, HSKM-B Lysine N-methyltransferase 3C, KMT3C
Substrates
Histone H3, 5 µMSAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N⁶-[methyl-³H]-L-lysine
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
SAH526
Sinefugin15,000
Scientific Information
Q9NRG4 (http://www.uniprot.org/uniprot/Q9NRG4)
Screening Location
Malvern, USA
Reference compound IC50 for SMYD2
Reference compound IC50 for SMYD2