Activity-based SMYD3 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Histone-lysine N-methyltransferase SMYD3, SET and MYND domain-containing protein 3, Zinc finger MYND domain-containing protein 1, ZMYND1
MEKK2, 0.5 µMSAM, 1 µM
S-adenosyl-L-[methyl-³H]methionine + Protein L- lysine = S-adenosyl-L-homocysteine + Protein [methyl-³H]-L- lysine
Reference Compound IC50s
Reference compound IC50 for SMYD3