SMYD3 Methyltransferase Assay Service

Activity-based SMYD3 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
SMYD3
Enzyme
Human SMYD3
Synonym
Histone-lysine N-methyltransferase SMYD3, SET and MYND domain-containing protein 3, Zinc finger MYND domain-containing protein 1, ZMYND1
Substrates
MEKK2, 0.5 µMSAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + Protein L- lysine = S-adenosyl-L-homocysteine + Protein [methyl-³H]-L- lysine
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
SAH17,000
EPZ0316863.5
Sinefungin>100,000
Suramin4,800
Scientific Information
Q9H7B4 (http://www.uniprot.org/uniprot/Q9H7B4)
Screening Location
Malvern, USA
Reference compound IC50 for SMYD3
Reference compound IC50 for SMYD3