SNARK Kinase Activity Assay Service

Activity-based SNARK kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology

Target Overview

RBE Target ID

SNARK

Enzyme

Human SNARK, wt

HGNC

NUAK2

Manning ID

NuaK2

Synonym

DKFZp434J037 (predicted protein only!), NUAK2, DKFZp434J037, SNARK, SNARK/NUAK2

UniProt

Q9H093 (NUAK2_HUMAN)

Kinase Type

Protein kinase

Kinase Group

CAMK

Assay Properties

Assay Format

33PanQinaseTM

Substrates

Histon H2B, ATP

Reaction

Histon H2B + 33P-ATP = 33P-Histon H2B + ADP

Readout

Enzymatic activity

Technical Reference Compound IC50 value(s)
Compounds IC50 (nM)
Staurosporine 5
Sorafenib 10000
Sunitinb 4000

Screening Location

Freiburg, Germany

Further Information

More information can be found on our website Kinase Assays

Service and Product Availability

Screening Opportunities
FreeChoice SNARK
345 wt Profiler Yes
345 IC50 wt Profiler Yes
Mutant Profiler Addon No
Lipid Kinase Profiler Addon No
IC50 Profiler No
Recombinant Protein Availability
Active recombinant protein

Yes; ProQinaseTM – SNARK
(Activity verified by 33PanQinaseTM-Assay)

Product Number

0414-0000-1

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