SNARK/NUAK2 Kinase Assay Service

Activity-based SNARK/NUAK2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
SNARK
Enzyme
Human SNARK
HGNC
NUAK2
Synonym
SNARK, DKFZp434J037
Substrates
Peptide substrate, [KKKVSRSGLYRSPSMPENLNRPR], 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
Staurosporine6.4
Ro 31-822014
GW50748,900
H-89550
Rottlerin17,800
Scientific Information
Q9H093 (http://www.uniprot.org/uniprot/Q9H093)
Screening Location
Malvern, USA
Reference compound IC50 for SNARK/NUAK2
Reference compound IC50 for SNARK/NUAK2