SNARK/NUAK2 Kinase Assay Service

Activity-based SNARK/NUAK2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
SNARK
Enzyme
Human SNARK
HGNC
NUAK2
Synonym
SNARK, DKFZp434J037
Scientific Information
Q9H093 (http://www.uniprot.org/uniprot/Q9H093)
Assay Properties
Substrates
Peptide substrate, [KKKVSRSGLYRSPSMPENLNRPR], 20 µM
ATP 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 1.9
Ro 31-8220 30
GW5074 15,000
H-89 280
Rottlerin >100,000
Screening Location
Malvern, PA, USA
Reference compound IC50 for SNARK/NUAK2