Activity-based SNARK/NUAK2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
SNARK
Enzyme
Human SNARK
HGNC
NUAK2
Synonym
SNARK, DKFZp434J037
Scientific Information
Q9H093 (http://www.uniprot.org/uniprot/Q9H093)
Assay Properties
Substrates
Peptide substrate, [KKKVSRSGLYRSPSMPENLNRPR], 20 µM
ATP 10 µM
ATP 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 1.9 |
| Ro 31-8220 | 30 |
| GW5074 | 15,000 |
| H-89 | 280 |
| Rottlerin | >100,000 |
Screening Location
Malvern, PA, USA
Reference compound IC50 for SNARK/NUAK2
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