SOS1 Binding Assay Service (Thermal shift)

KRAS-related Assays: SOS1 binding assay service for compound screening and profiling using the thermal shift assay format to measure compound binding to the target

Target

Human SOS1

HGNC

SOS1

Protein

SOS1 (aa 564-1069), strep tagged. Expressed in Ecoli.

Synonyms

Son of sevenless homolog 1, GF1, GGF1, GINGF, HGF, NS4

Scientific information

SOS1: Q07889 (https://www.uniprot.org/uniprot/Q07889)

Screening location

Malvern, PA, US

Reaction

Compound binding to SOS1

Readout

Protein stability change due to ligand binding via thermal shift assay

Concentration of protein in assay

0.15 mg/ml (~2.5 µM)

Concentration of compound in assay

25 µM

Apo form Tm (°C)

43.5

Reference Compound ΔTms

BAY-293 +3 (pink) , no stabilization was observed in the presence of other control compounds (ARS-1620, AMG-510, BI-2852)

Melt peak of SOS1 protein at increasing temperatures in presence of compounds BAY-293 (pink), ARS-1620 (green), AMG-510 (blue) or BI-2852 (brown) or none (black)

SOS1_thermalshift_RAS_Malvern