SOS2 Binding Assay Service (Thermal shift)

KRAS-related Assays: SOS2 binding assay service for compound screening and profiling using the thermal shift assay format to measure compound binding to the target

Target

Human SOS2

HGNC

SOS2

Protein

SOS2 (aa 562-1047), strep tagged. Expressed in Ecoli.

Synonyms

Son of sevenless homolog 2

Scientific information

SOS2: Q07890 (https://www.uniprot.org/uniprot/Q07890)

Screening location

Malvern, PA, US

Reaction

Compound binding to SOS2

Readout

Protein stability change due to ligand binding via thermal shift assay

Concentration of protein in assay

Typically tested at 0.1 mg/ml

Concentration of compound in assay

n/a

Apo form Tm (°C)

43.5

Reference Compound ΔTms

No reference compound is currently available for SOS2. No protein stabilization was observed when tested with reference compounds BAY293, ARS-1620, AMG-510 or BI-2852.

Melt peak of SOS2 protein at increasing temperatures in presence of compounds BAY-293 (pink), ARS-1620 (green), AMG-510 (blue) or BI-2852 (brown) or none (black)

SOS2_thermalshift_RAS_Malvern
SOS2_thermalshift_RAS_Malvern