SP100 Bromodomain Assay Service (ThermoShift)

SP100-His Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.

Target Overview
CAT#

Tm-SP100-H

Protein

Human SP100 bromodomain, His-tagged

Synonyms

Nuclear dot-associated Sp100 protein

Assay Properties
Readout

Thermal Stability Assay (TSA)

Concentration of protein in assay

0.125 mg/ml (5.2 µM)

Concentration of compound in assay

25 µM

Apo form Tm (°C)

51,5

Reference Compound ΔTms:

No stabilization was observed in the presence of control compounds (JQ1, PFI1, CBP112, Bromosporine, SGC-CBP30, BET151, RVX-208, GSK2801 and PFI3)

Scientific Information

P23497 (http://www.uniprot.org/uniprot/P23497)

Screening Location

Malvern, USA

Further information

More information can be found on our website Reader Domain Assay Services.

Melt Curve

Melt Curve

Melt Peak

Melt Peak