SP110c Bromodomain Assay Service (ThermoShift)

SP110c-GST Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.

Target Overview
CAT#

Tm-SP110c-GST

Protein

Human SP110c bromodomain, GST-tagged

Synonyms

Interferon-induced protein 41/75, Speckled 110 kDa, Transcriptional coactivator Sp110

Assay Properties
Readout

Thermal stability change after ligand binding

Concentration of protein in assay

0.1 mg/ml (2.1 µM)

Concentration of compound in assay

25 µM

Apo form Tm (°C)

45,5

Reference Compound ΔTms:

No stabilization was observed in the presence of control compounds (JQ1, PFI1, CBP112, Bromosporine, SGC-CBP30, BET151, RVX-208, GSK2801 and PFI3)

Scientific Information

Q9HB58 (http://www.uniprot.org/uniprot/Q9HB58)

Screening Location

Malvern, USA

Further information

More information can be found on our website Reader Domain Assay Services.

Melt Curve

Melt Curve

Melt Peak

Melt Peak