Activity-based SPHK1 lipid kinase assay for compound screening and profiling via ADP-Glo kinase assay.
Target Overview
CAT#
SPHK1
Enzyme
Human SPHK1
HGNC
SPHK1
Synonym
Sphingosine kinase 1
Substrates
Sphingosine, 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Sphingosine + ATP = Sphingosine 1-phosphate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| PF-543 | 30 |
Scientific Information
Q9NYA1 (http://www.uniprot.org/uniprot/Q9NYA1)
Screening Location
Malvern, USA
Reference compound IC50 for SPHK1
PDF