SPHK2 Kinase Assay Service

Activity-based SPHK2 lipid kinase assay for compound screening and profiling via ADP-Glo kinase assay.
Target Overview

CAT#
SPHK2

Enzyme
Human SPHK2

HGNC
SPHK2

Synonym
Sphingosine kinase 2

Substrates
Sphingosine, 50 µM
ATP 10 µM

Assay Properties

Reaction
Sphingosine + ATP = Sphingosine 1-phosphate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
PF-543 36,000
Scientific Information
Q9NRA0 (http://www.uniprot.org/uniprot/Q9NRA0)

Screening Location
Malvern, USA

Reference compound IC50 for SPHK2
Reference compound IC50 for SPHK2