Activity-based SRPK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
SRPK1
Enzyme
Human SRPK1
HGNC
SRPK1
Synonym
SFRSK1
Substrates
Peptide substrate, [GRSRSRSRSR], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 120 |
| Ro 31-8220 | 680 |
| GW5074 | 7,300 |
| Rottlerin | 2,000 |
Scientific Information
Q96SB4 (http://www.uniprot.org/uniprot/Q96SB4)
Screening Location
Malvern, USA
Reference compound IC50 for SRPK1
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