Activity-based SSTK/TSSK6 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
SSTK/TSSK6
Enzyme
Human SSTK/TSSK6
HGNC
TSSK6
Synonym
FLJ24002, SSTK
Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 160 |
| Ro 31-8220 | 7,400 |
| GW 5074 | 65,000 |
| H-89 | >100,000 |
| Rottlerin | >100,000 |
Scientific Information
Q9BXA6 (http://www.uniprot.org/uniprot/Q9BXA6)
Screening Location
Malvern, USA
Reference compound IC50 for SSTK/TSSK6
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