Activity-based STK22D/TSSK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
STK22D/TSSK1
Enzyme
Human STK22D/TSSK1
HGNC
TSSK1B
Synonym
TSSK1, FKSG81, SPOGA4, STK22D
Substrates
Peptide substrate, [KKKVSRSGLYRSPSMPENLNRPR], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | <0.1 |
| Ro 31-8220 | 1,200 |
| GW 5074 | 7,500 |
| H-89 | 4,900 |
Scientific Information
Q9BXA7 (http://www.uniprot.org/uniprot/Q9BXA7)
Screening Location
Malvern, USA
Reference compound IC50 for STK22D
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