Activity-based STK25/YSK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
STK25
Enzyme
Human STK25
HGNC
STK25
Synonym
YSK1, SOK1
Substrates
MBP (Myelin Basic Protein), 10 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 23.6 |
| Ro 31-8220 | 150 |
| GW5074 | 35,000 |
| H-89 | 57,000 |
Scientific Information
O00506 (http://www.uniprot.org/uniprot/O00506)
Screening Location
Malvern, USA
Reference compound IC50 for STK25/YSK1
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