Activity-based STK39/STLK3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
STK39/STLK3
Enzyme
Human STK39/STLK3
HGNC
STK39
Synonym
STLK3, DCHT, SPAK
Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 22 |
| Ro 31-8220 | 420 |
| GW 5074 | 3,700 |
| H-89 | 23,000 |
| Rottlerin | >100,000 |
Scientific Information
Q9UEW8 (http://www.uniprot.org/uniprot/Q9UEW8)
Screening Location
Malvern, USA
Reference compound IC50 for STK39/STLK3
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