SUV39H1 Methyltransferase Assay Service

Activity-based SUV39H1 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
SUV39H1
Enzyme
Human SUV39H1
Synonym
Histone-lysine N-methyltransferase SUV39H1, Suppressor of variegation 3-9 homolog 1, Su(var)3-9 homolog 1, Position-effect variegation 3-9 homolog, Histone H3-K9 methyltransferase 1, H3-K9-HMTase 1, Lysine N-methyltransferase 1A, KMT1A, SUV39H, SUV91
Substrates
Histone H3, 5 µMSAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N⁶-[methyl-³H]-L-lysine
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
SAH23,000
Sinefungin140,000
Chaetocin330
Scientific Information
O43463 (http://www.uniprot.org/uniprot/O43463)
Screening Location
Malvern, USA
Reference compound IC50 for SUV39H1
Reference compound IC50 for SUV39H1