SUV420H1-tv2 Methyltransferase Assay Service

Activity-based SUV420H1-tv2 Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
SUV420H1-tv2
Enzyme
Human SUV420H1-transcription variant 2
Synonym
Histone-lysine N-methyltransferase SUV420H1, Lysine N-methyltransferase 5B Suppressor of variegation 4-20 homolog 1, KMT5B
Substrates
Oligonucleosomes, 0.05 mg/mlSAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N⁶-[methyl-³H]-L-lysine
Readout
Enzymatic activity
Reference Compound IC50s
CompoundsIC50 (nM)
SAH5,400
Sinefungin130,000
BIX 01294>200,000
Chaetocin33,000
Scientific Information
Q4FZB7-2 (http://www.uniprot.org/uniprot/Q4FZB7)
Screening Location
Malvern, USA
Reference compound IC50 for SUV420H1-tv2
Reference compound IC50 for SUV420H1-tv2