TAF1-2 Bromodomain Assay Service (ThermoShift)

TAF1-2-GST Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.

Target Overview
CAT#

Tm-TAF1-2-GST

Protein

Human TAF1-2 bromodomain, GST-tagged

Synonyms

Transcription initiation factor TFIID subunit 1; p250, TAFII-250, Cell cycle gene 1 protein

Assay Properties
Readout

Thermal stability change after ligand binding

Concentration of protein in assay

0.1 mg/ml (2.3 µM)

Concentration of compound in assay

25 µM

Apo form Tm (°C)

48,5

Reference Compound ΔTms:

Bromosportine - 2 (Blue) No stabilization was observed in the presence of other control compounds (JQ1, PFI1, CBP112, SGC-CBP30, BET151, RVX-208, GSK2801 and PFI3)

Scientific Information

P21675 (http://www.uniprot.org/uniprot/P21675)

Screening Location

Malvern, USA

Further information

More information can be found on our website Reader Domain Assay Services.

Melt Curve

Melt Curve

Melt Peak

Melt Peak