TAF1L Bromodomain Assay Service (ThermoShift)

TAF1L-Tndm-GST Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.

Target Overview




Human TAF1L-T bromodomain, GST-tagged


TAF(II)210, TBP-associated factor 1-like, TBP-associated factor 210 kDa, Transcription initiation factor TFIID 210 kDa subunit

Assay Properties


Thermal stability change after ligand binding

Concentration of protein in assay

0.1 mg/ml (1.7 µM)

Concentration of compound in assay

25 µM

Apo form Tm (°C)


Reference Compound ΔTms:

GSK2801 – 1.5 (Yellow)No stabilization was observed in the presence of other control compounds (JQ1, PFI1, CBP112, Bromosporine, SGC-CBP30, BET151, RVX-208, and PFI3)

Scientific Information

Q8IZX4 (http://www.uniprot.org/uniprot/Q8IZX4)

Screening Location

Malvern, USA

Further information

More information can be found on our website Reader Domain Assay Services.

Melt Curve

Melt Curve

Melt Peak

Melt Peak